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Journal of Genetic Engineering and Biotechnology
Fig. 9 | Journal of Genetic Engineering and Biotechnology

Fig. 9

From: Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison

Fig. 9

HIP expression of HF7 versus CL4 recombinant clones. A Comparison of expression in BMMY medium. HF7 and CL4 were replicated three times in one batch. M = Precision plus protein™ dual-color standard (Bio-Rad). B A comparison of the HIP expression in the BSMM medium. Lane 1–3 = three replicates of HF7, lane 4–6 = three replicates of CL4, lane 7–10 = USP human insulin standard (0.25, 0.125, 0.0625, 0.03125 mg/mL, respectively). C HI-USP standard regression analysis. D Two-way ANOVA analysis of HIP’s HF7 and CL4 concentration in BMMY and BSMM media. According to analysis (p < 0.05), the asterisk (*) indicates significant differences in HIP concentration between HF7 and CL4 in both media

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