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Journal of Genetic Engineering and Biotechnology
Fig. 8 | Journal of Genetic Engineering and Biotechnology

Fig. 8

From: Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison

Fig. 8

SDS-PAGE of methanol optimization. A The 72-h culture supernatant of HF7 clone with different concentrations of methanol induction. Lanes 2–8 are HIP expressions at 0, 0.5, 1, 2, 3, 4, and 5% methanol, respectively. HI = human insulin standard (1.82 mg/mL). B ANOVA analysis of HIP expressions at different methanol concentrations, p value < 0.05 indicates that the means in all groups are significantly different

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