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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison

Fig. 3

Genetic validation of nine recombinant P. pastoris (HF) clones. A PCR validation method. M = 1 kb DNA ladder; Lane 2–10 = amplicon from nine HF genomes, P = pD902-IP-full-length-α-factor plasmid as a template; WT = wild-type genome. B Multiple sequence alignment of nine recombinant HF clones

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