Fig. 10From: Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparisonWestern blot analysis. A SDS-PAGE of the HF7 and CL4-secreted protein showed the HIP. B The PVDF membrane after blotting. HF7 = HIP secreted from a full-length α-factor clone, CL4 = HIP secreted from a truncated α-factor clone, M = Protein marker, HI = USP human insulin standardBack to article page