Journal of Genetic Engineering and Biotechnology

Table 1 Callus induction and regeneration media used for the optimization of the regeneration system of sudangrass

From: Development of an in vitro regeneration system from immature inflorescences and CRISPR/Cas9-mediated gene editing in sudangrass

Component	Callus induction media^*				Regeneration media^**
Component	M1	M2	M3	M4	Shooting (S)	Rooting (R)
MS basal (g/L)	4.4	4.4	4.4	4.4	4.4	4.4
MES (mg/L)	500	0	0	500	0	0
CuSO₄ (mg/L)	0	800	0	0	0	0
Glycine (mg/L)	0	0	0	7	0	0
L-proline (mg/L)	700	700	2400	0	700	700
L-asparagine (mg/L)	0	0	0	100	0	0
Myo-inositol (mg/L)	100	150	0	100	100	100
Nicotinic acid (mg/L)	0.5	0	0	1	0.5	0.5
Pyridoxine-HCl (mg/L)	0.5	0	0	1	0.5	0.5
Thiamine-HCl (mg/L)	10	0	0	1	2.5	2.5
Ascorbic acid (mg/L)	0	1	0	0	10	10
2,4-D (mg/L)	1.5	1	4	2.5	0	0
IAA (mg/L)	0	0	0	0	0	1
kinetin (mg/L)	0	0	0.5	0.5	1	1
Zeatin (mg/L)	0	0	0	0	0.5	0
TDZ (mg/L)	0	0	0	0	0.1	0
BAP (mg/L)	0	0.5	0	0	0	0
ABA (mg/L)	0	0	0	0	0.25	0
Charcoal (mg/L)	0	0	0	0	400	400
PVP (mg/L)	0	0	0	0	300	300
Peptone (g/L)	0	0.82	0	0	0	0
Maltose (g/L)	0	30	0	0	0	0
Sucrose (g/L)	20	0	45	20	30	30

^*Callus induction media: M1 [22], M3 [30], and M4 modified [8]
^**Regeneration media: (S and R) [22]

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