Fig. 5

Sanger sequencing electropherogram of purified PCR products from the target sequence of the CRISPR/Cas9 system. A Sequencing was carried out using COMT_Exon1 primers to target gRNA-392; C_COMT1 is a control plant negative for the CRISPR/Cas9 system, while 1_COMT1, 2_COMT1, and 3_COMT1 are transgenic plants positive for the CRISPR/Cas9 system. B Sequencing was carried out using COMT_Exon2 primers to target gRNA-79; C_COMT2 is a control plant negative for the CRISPR/Cas9 system, while 1_COMT2, 2_COMT2, and 3_COMT2 are transgenic plants positive for the CRISPR/Cas9 system. The red border denotes the PAM site, and the yellow border denotes the gRNA target site substitution. Sequencing chromatograms indicate the mutation in the target sequences, resulting in the variety of peaks in the chromatograms. The position of the gRNA is highlighted in blue