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Journal of Genetic Engineering and Biotechnology
Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: Development of an in vitro regeneration system from immature inflorescences and CRISPR/Cas9-mediated gene editing in sudangrass

Fig. 4

PCR reactions carried out for the Cas9 ∼260bp (A) and NPTII ∼224bp (B) genes and 35S promoter ∼189bp (C) in transgenic T0 plants. (M) 1kb molecular weight size marker; (C) control (nontransformed plant); (+Ve) positive amplification control of the vector; Lanes (1:5) are DNA samples from individual events sudangrass resulting from transformation by biolistic particle delivery systems

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