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Journal of Genetic Engineering and Biotechnology

Table 2 Comparison of concentration L1 HPV 52 and protein recovery

From: Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52

Step

Total protein (mg)

ELISA (ug)

Hp

Pp

Hp

Pp

Lysate supernatants

76.49

73.83

630

840

(NH)2SO4 precipitation

14.62

14.23

Recovery (%)

19.11

19.27

4.31

5.90

  1. Total protein was determined by BCA protein assay. Hp H. polymorpha, Pp P. pastoris. % protein recovery was determined by comparing ELISA and (NH)2 SO4 precipitation values
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