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Table 2 The kinetic parameters for the purified B. subtilis MK775302

From: The catalytic and kinetic characterization of Bacillus subtilis MK775302 milk clotting enzyme: comparison with calf rennet as a coagulant in white soft cheese manufacture

Kinetic parameter

50 °C

60 °C

70 °C

t1/2 (min)

385

135.9

86.5

Thermal deactivation constant (kd) (min)

1.8 × 10−3

5.1 × 10−3

8.01 × 10−3

(D) value min

1279.4

451.6

287.8

Activation energy Ea (kJ/mole)

47.77

Deactivation energy for irreversible thermal inactivation

Ed (kJ/mole)

173.5