Table 1 Partial purification of crude B. subtilis MK775302 MCE
Acetone concentration (%) | Protein content mg/fraction | Activity of fraction (U) | Specific activityU/mg protein | Recovered protein (%) | Recovered activity (%) | Fold purification |
|---|---|---|---|---|---|---|
Culture filtrate | 1920 | 60,000 | 31.3 | 100 | 100 | 1.00 |
25 | 70.38 | 5532.6 | 78.6 | 3.66 | 9.22 | 2.5 |
50 | 158 | 27,790 | 175.8 | 8.2 | 46.3 | 5.6 |
75 | 140.52 | 10,512 | 74.8 | 7.32 | 17.5 | 2.4 |