Table 2 The summary of pectinase production and fungal growth by Aspergillus niger LFP-1 after improvement of physicochemical parameters in a shake flask system

Incubation time (days)

8

6

Incubation temperature (⁰C)

30

30

pH

4.5

4.5

Agitation speed (rpm)

100

150

Inoculum size (spores/mL)

1 × 10⁷

1 × 10⁶

Carbon sources

pectin

pectin

Concentration of pectin (%)

1.0

2.5

Nitrogen sources

ammonium sulfate

ammonium nitrate

Concentration of NH₄NO₃

0.1

0.4

Pectinase activity (U/mL)

0.88 ± 0.09

7.41 ± 0.24

Increment (%)

-

88.12

Fungal growth (g/L)

2.47 ± 0.14

4.62 ± 0.13

Increment (%)

-

46.54