Journal of Genetic Engineering and Biotechnology

Table 3 Effect of organic solvents on HAL1 protease activity

From: Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate

Metal ion	Relative activity (%)
Metal ion	5 mM	10 mM
No Metal	100	100
Fe2 +	37.3 ± 0.6	35.5 ± 0.9
Cd2 +	45.1 ± 0.7	38 ± 0.9
Cu2 +	95.4 ± 0.3	56.5 ± 0.9
Ca2 +	111.7 ± 0.4	127.7 ± 1.4
Mg2 +	112.3 ± 0.8	120.6 ± 0.1
Pb²⁺	98.6 ± 0.2	92.5 ± 0.9
Zn²⁺	43.1 ± 0.6	31.9 ± 0.3
Mn²⁺	905.1 ± 4.1	1165.8 ± 4.7

Data are means ± standard deviations (SD) for three replicates

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