Fig. 2
From: Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system
RNA-guided DNA-transposition process [47]. 1-Cas8 of the INTEGRATE system recognizes PAM sequences in double-stranded target DNA. 2- crRNA invades the dsDNA target to form RNA: DNA hybrid with the complementary strand, displacing the non-complementary strand to form the R-loop structure. Subsequently, TniQ, bound to the PAM distal end of the DNA-bound cascade complex, is expected to recruit TnsC, TnsA, and TnsB to activate RNA-guided DNA transposition