Table 4 Physicochemical properties of the protein identified with ExPasy ProtParam server

Amino acid composition (number of residues)

Ala (54), Arg (1), Asn (5), Asp (10), Gln (2), Glu (26), Gly (23), His (1), Ile (4), Leu (18), Lys (32), Met (6), Phe (5), Pro (10), Ser (12), Thr (9), Trp (6), Tyr (15), Val (15)

Total number of amino acids

254

Molecular weight

26,888.54

Theoretical pI

5.54

Total number of negatively charged residues (Asp + Glu)

36

Total number of positively charged residues (Arg + Lys)

33

Instability index

The instability index (II) is computed to be 20.34. This classifies the protein as stable

Aliphatic index

72.17

Grand average of hydropathicity (GRAVY)

− 0.303