Estimation of genetic diversity and population genetic structure in Gymnema sylvestre (Retz.) R. Br. ex Schult. populations using DAMD and ISSR markers

Vaishnav, Kanchana; Tiwari, Vandana; Durgapal, Anjala; Meena, Baleshwar; Rana, T. S.

doi:10.1186/s43141-023-00497-7

Journal of Genetic Engineering and Biotechnology

Table 2 Details of DAMD and ISSR markers and the polymorphism obtained with these markers

From: Estimation of genetic diversity and population genetic structure in Gymnema sylvestre (Retz.) R. Br. ex Schult. populations using DAMD and ISSR markers

Marker	Sequence (5′ → 3′)	AT (°C)	TL	ML	PL	PPL	Approx. loci size (bp)	PIC	Rp	DI	EMR	MI
DAMD
14C2	GGCAGGATTGAAGC	50	17	3	14	82.35	350–1200	0.20	21.58
33.6	AGGGCTGGAGG	50	21	2	19	90.48	170–1100	0.29	27.05
FvIIex8	ATGCACACACACAGG	55	21	6	15	71.43	230–1000	0.23	30.51
FvIIex8C	CCTGTGTGTGTGCAT	55	19	3	16	84.21	275–1300	0.30	25.19
HBV	GGTGTAGAGAGGGGT	50	17	2	15	88.24	360–1200	0.24	21.44
HVR	CCTCCTCCCTCCT	50	19	4	15	78.95	310–1300	0.22	22.81
URP9F	ATGTGTGCGATCAGTTGCTG	55	18	3	15	83.33	200–1100	0.23	24.61
URP38F	AAGAGGCATTCTACCACCAC	55	23	4	19	82.61	200–1600	0.22	37.86
Total (8)			155	27	128		170–1600
Average/marker			19.38	3.38	16	82.58		0.24	26.38	0.29	13.21	3.88
ISSR
UBC-808	AGAGAGAGAGAGAGAGC	52	13	2	11	84.62	240–1400	0.12	23.17
UBC-809	AGAGAGAGAGAGAGAGG	54	17	5	12	70.59	290–1000	0.22	21.34
UBC-810	GAGAGAGAGAGAGAGAT	50	13	2	11	84.62	300–1000	0.29	15.83
UBC-812	GAGAGAGAGAGAGAGAA	50	16	5	11	68.75	240–1000	0.20	23.95
UBC-823	TCTCTCTCTCTCTCTCC	50	19	4	15	78.95	280–1500	0.20	28.83
UBC-825	ACACACACACACACACT	50	15	2	13	86.67	300–1100	0.22	20.25
UBC-826	ACACACACACACACACC	58	20	3	17	85.00	350–1250	0.26	30.35
UBC-830	TGTGTGTGTGTGTGTGG	58	18	3	15	83.33	300–1200	0.32	24.13
UBC-835	AGAGAGAGAGAGAGAGYC	50	22	3	19	86.36	300–1500	0.27	30.83
UBC-836	AGAGAGAGAGAGAGAGYA	54	15	2	13	86.67	290–820	0.24	20.78
UBC-840	GAGAGAGAGAGAGAGAYT	50	11	1	10	90.91	300–900	0.21	13.05
UBC-841	GAGAGAGAGAGAGAGAYC	51	16	3	13	81.25	270–1200	0.26	19.51
UBC-842	GAGAGAGAGAGAGAGAYG	54	18	4	14	77.78	190–950	0.33	23.91
UBC-860	TGTGTGTGTGTGTGTGRA	54	19	1	18	94.74	350–1200	0.28	21.46
UBC-866	CTCCTCCTCCTCCTCCTC	55	12	6	06	50.00	500–1200	0.22	19.12
UBC-868	GAAGAAGAAGAAGAAGAA	53	14	4	10	71.43	310–1000	0.18	21.66
UBC-887	DVDTCTCTCTCTCTCTC	50	16	5	11	68.75	300–1000	0.23	23.29
Total (17)			274	55	219		190–1500
Average/marker			16.12	3.24	12.88	79.93		0.24	22.44	0.30	10.30	3.14
Cumulative (25)			429	82	347	80.89	170–1600	0.24	24.41	0.30	11.76	3.51

TL total number of loci amplified, ML monomorphic loci, PL polymorphic loci, PPL percentage of polymorphic loci, Approx. approximately, bp base pairs, PIC polymorphic information content, Rp resolving power, DI diversity index, EMR effective multiplex ratio, MI marker index. Mixed bases; D = A or G or T not C; R = A or G; V = A or C or G not T; Y = C or T

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