Table 1 Summary of steps employed in the purification of l-asparaginase from Trichoderma viride culture filtrate
Purification step | Total activity (U) | Total protein (mg) | Sp. activity (U/mg protein) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 4597 ± 154 | 238 ± 11.3 | 19.3 | 100 | 1.0 |
Ethanol precipitation (50%) | 2863 ± 58.2 | 31.4 ± 2.15 | 91.2 | 62.3 | 4.7 |
Dialysis | 2798 ± 36.8 | 29.4 ± 2.13 | 95.2 | 60.9 | 4.9 |
Sephadex G-100 | 2148 ± 23.5 | 6.4 ± 0.17 | 335.6 | 46.7 | 17.4 |
Sephadex G-200 | 1789 ± 14.7 | 2.6 ± 0.12 | 688.1 | 38.9 | 35.7 |