Table 1 Secondary metabolite constituents of Spondias mombin leaf
Secondary metabolites | Observation | Concentration (mg/ml) |
|---|---|---|
Alkaloids | Cream colour with Mayer’s reagent; reddish-brown with Wagner’s reagent | 3.40 ± 0.10 |
Anthraquinones | Absence of bright pink colour with NH3 | Not detected |
Cardenolides and dienolides | Absence of turbid brown ring at the interface | Not detected |
Cardiac glycosides | Brown-violet ring at the interface | 0.01 ± 0.00 |
Chalcone | Absence of red coloration with concentrated sulphuric acid | Not detected |
Phlobatannins | Absence of reddish precipitate with HCl | Not detected |
Saponins | Stable, persistent froth with distilled water | 4.80 ± 0.35 |
Steroids | Presence of violet to blue or green colour with H2SO4 | 0.92 ± 0.09 |
Tannins | Greenish-brown precipitate | 1.47 ± 0.06 |
Terpenoids | Absence of reddish-brown colour with H2SO4 | Not detected |
Total flavonoids | Dark yellow precipitate with NH3 | 2.80 ± 0.36 |
Total phenolics | Greenish precipitate with FeCl3 | 1.73 ± 0.19 |