Purification, characterization, and enzyme kinetics of a glutathione S transferase from larvae of the camel tick Hyalomma dromedarii

Journal of Genetic Engineering and Biotechnology

Table 3 Effect of inhibitors on the purified TLGST

Inhibitor	Concentration (Mm)	Inhibition %
Control	–	0.0
p-Chloromercuribenzoic acid (pCMB)	1 mM	97.2
p-Hydroxymercuribenzoate (pHMB)	5 mM	21.2
N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK)	5 mM	74.8
Dithiothreitol (DTT)	5 mM	10.4
EDTA	5 mM	37.9
β-Mercaptoethanol	5 mM	10.7
Iodoacetamide	5 mM	63
Potassium cyanide	5 mM	2.1
Quercetin	5 mM	88.6
Cumene hydroperoxide	5 mM	16.5
Hematin	5 mM	42.7
Lithocholic acid	5 mM	30.0
Triphenyltin chloride	5 mM	42.7