Table 1 A typical purification scheme of the camel tick larvae GST (TLGST)
Purification step | Total protein (mg) | Activity (unit) | Specific activity | Recovery (%) | Fold purification |
|---|---|---|---|---|---|
Camel tick larvae crude extract | 322 | 12.9 | 0.04 | 100 | 1.0 |
40–80% (NH4)2SO4 fraction | 118 | 10.7 | 0.09 | 82.9 | 2.3 |
Glutathione Sepharose fraction | 11.6 | 6.6 | 0.57 | 51.0 | 14.3 |
Sephacryl S-300 fraction | 2.7 | 4.2 | 1.56 | 32.5 | 39.0 |