Table 1 List of ISSR primers utilized in this study, their sequences, annealing temperatures, number of bands produced by each primer, and polymorphism percentage
# | Primer name | Sequence (5′-3′) | Annealing temperature (ºC) | Primer length (bp) | Number of bands | Band type | Polymorphism (%) | ||
|---|---|---|---|---|---|---|---|---|---|
P | M | U | |||||||
1 | 808 | AGAGAGAGAGAGAGAGC | 59 | 17 | 5 | 3 | 1 | 1 | 60.0% |
2 | 827 | ACACACACACACACACG | 62 | 17 | 6 | 3 | 2 | 1 | 50.0% |
3 | HB-15 | GTG GTGGTG GC | 57 | 11 | 8 | 1 | 4 | 3 | 12.5% |
4 | 829 | TGTGTGTGTGTGTGTGC | 62 | 17 | 10 | 6 | 2 | 2 | 60.0% |
5 | HB-8 | GAGAGAGAGAGAGG | 44 | 14 | 5 | 3 | 2 | 0 | 60.0% |
6 | CAG | CAGCAGCAGCAGCAGCAG | 56 | 18 | 6 | 4 | 1 | 1 | 66.6% |
7 | 17898B | CACACACACACAGT | 42 | 14 | 11 | 5 | 3 | 3 | 45.5% |
| Â | Total | Â | Â | 51 | 25 | 15 | 11 | Â | |