Extract of Bletilla formosana callus elevates cellular antioxidative activity via Nrf2/HO-1 signaling pathway and inhibits melanogenesis in zebrafish

Journal of Genetic Engineering and Biotechnology

Table 1 Primers used for real-time quantitative PCR

Genes		Sequence (5'-3')	Accession Number	Ref
GAPDH	Forward	ACTTCAACAGCGACACCCAC	NM_002046.7	[23]
GAPDH	Reverse	CCCTGTTGCTGTAGCCAAATTC
Nrf2	Forward	CTGCCAACTACTCCCAGGTT	NM_006164.5
Nrf2	Reverse	AAGTGACTGAAACGTAGCCGA
CAT	Forward	AAGGTTTGGCCTCACAAGGA	NM_001752.4	[24]
CAT	Reverse	GCGGTAGGGACAGTTCACAG
SOD	Forward	TGGGCAATGTGACTGCTGAC	NM_000454.5	[25]
SOD	Reverse	ACCAGCCAAACGACTTCCAG
GPx1	Forward	GTTTGGGCATCAGGAGAACG	NM_000581.4
GPx1	Reverse	CAACATCGTTGCGACACACC
HO-1	Forward	CTCCACATCCAGCTCTTTGAGG	NM_002133.3
HO-1	Reverse	CGTGGGCAGAATCTTGCAC
NQO1	Forward	CTGAAAGGCTGGTTTGAGCG	NM_000903.3
NQO1	Reverse	TCCACTCTGAATTGGCCAGAG