The potential role of the KFG and KITLG proteins in preventing granulosa cell apoptosis in Bubalus bubalis

Journal of Genetic Engineering and Biotechnology

Table 1 Oligonucleotide primer sequences and amplicon size used for real-time PCR

Gene	Primer sequence 5′-3′	Size (bp)	Accession number
18S	Frd-AGAAACGGCTACCACATCCAA	140	AF176811.1
18S	Rev-CGGAAGGATTTAAAGTGGACTCA	140	AF176811.1
Fas	Frd-CTGTTTCTGGACCATTGT	92	NM_174662.2
Fas	Rev-GGAGTTCGCTTCAGTAAT	92	NM_174662.2
FasL	Frd-TGGAATGGGAAGACACCTATGG	120	NM_001098859.2
FasL	Rev-GACCCCGGAAGTACACTTTGG	120	NM_001098859.2
Inhibin α	Frd-CTGAGCCCGAGGACCAAGA	95	NM_174094.4
Inhibin α	Rev-CTCCTCAGCCTCTCCAGCAT	95	NM_174094.4
bFGF2	Frd-TTTCTTTTTTGAACGATTGGAGTCT	85	NM_174056.4
bFGF2	Rev-TCGTTTCAGTGCCACATACCA	85	NM_174056.4
c-kit	Frd-CAGAAATCCTGACGCATGACA	105	DQ314491.1
c-kit	Rev-GCTCGGTTCCTGGACAAAAG	105	DQ314491.1
FGFR2	Frd-CGGCCCTCCTTCAATTTAGTT	150	NM_001205310.1
FGFR2	Rev-ACTGATCATGGCGGCATCTC	150	NM_001205310.1
cFLIP	Frd-AGTCATCCATCAGGTAGAAGAAG	77	NM_001012281.1
cFLIP	Rev-AGCAACATCTCGGCACAA	77	NM_001012281.1
Bax	Frd-AAGAAGCTGAGCGAGTGT	78	NM_173894.1
Bax	Rev-AGCTGCGATCATCCTCTG	78	NM_173894.1
Bcl-2	Frd-ACCTGCACACCTGGATCCA	100	NM_001166486.1
Bcl-2	Rev-AGAGACAGCCAGGAGAAATCAAA	100	NM_001166486.1
Bcl-xL	Frd-AAGCGTAGACAAGGAGAT	76	NM_001077486.2
Bcl-xL	Rev-TAGGTGGTCATTCAGGTAA	76	NM_001077486.2

18S 18S ribosomal RNA gene, Fas Fas cell surface death receptor, FasL TNF superfamily, member 6, cFLIP Cellular FLICE-like inhibitory protein, Inhibin α Inhibin alpha, bFGF2 Basic Fibroblast growth factor 2, C-kit C-kit receptor, FGFR2 Fibroblast growth factor receptor 2, Bcl-xL B cell lymphoma-extra-large, Bcl2 B cell CLL/lymphoma 2, Bax BCL2-associated X protein