Fig. 4

Autocrine action of KGF and KITLG in buffalo granulosa cells. A A significant increase was observed for c-kit and FGFR2 expression in a dose-dependent manner of KGF treated cells. B The KITLG treated cells resulted in upregulation of c-kit (by > 10 folds) and FGFR2 (by > 5 folds) (B). These observations suggest that KGF and KITLG act synergistically by regulating each other’s expression across their site of production and action. K represents the KGF, KITLG is denoted by KL. The dose concentrations are defined as 10, 20, and 50 ng/ml for both KGF and KITLG. Values are expressed as the mean ± SD of three experiments; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control (non-treated)