Fig. 3

Treatment with KGF and KITLG enhances the growth of granulosa cells. The mRNA expressions in cultured granulosa cells in response to KGF and KITLG treatment were determined using Real-time PCR. Inhibin-α was consistently increased with doses dependent manner of KFG (A) and KITLG (B). The mRNA expression of bFGF remained almost unchanged at 10 and 20 ng/ml as compared to the control with both KGF (A) and KITLG (B) treatment and significantly upregulated at 50 ng/ml concentration. Upregulation for Inhibin-α and bFGF expression was more consistent and significant at the dose of 50 ng/ml as compared to that of 10 and 20 ng/ml. C Combined treatment of KGF and KITLG at 10 ng/ml resulted in increased expression of Inhibin-α and bFGF (> 10-fold), whereas there is no change at the dosage of 10 and 20 ng/ml. These observations suggest the role of KGF and KITLG in growth of granulosa cell. K represents the KGF, KITLG is denoted by KL and A is KGF + KITLG. The dose concentrations are defined as 10, 20, and 50 ng/ml for both KGF and KITLG. Values are expressed as the mean ± SD of three experiments; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control (non-treated)