Fig. 1

The relative expression of Fas and FasL mRNA in KGF and KITLG treated granulosa cells. Expression of Fas and FasL were significantly down regulated in a dose-dependent manner by KGF and KITLG treatment and was found to be lowest at the concentration of 50 ng/ml (A, B). Expression of cFLIP was significantly increased and was in direct proportion to the dosage of KGF and KITLG and was found to be maximum at the concentration of 50 ng/ml. When granulosa cells were treated with both KGF and KITLG, concentration of 10 ng/ml was found to be more significant in regulation of Fas-FasL pathway by cFLIP (C). Data are presented as relative expression of Fas, FasL, and cFLIP mRNA/18S mRNA normalized to untreated control values. K represents the KGF, KITLG is denoted by KL and A is KGF+KITLG. The dose concentrations are defined as 10, 20, and 50 ng/ml for both KGF and KITLG. Values are expressed as the mean ± SD of three experiments; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control (non-treated)