Fig. 2

Production of polyclonal antibody by the pseudotyped virus from immunized BALB/C mice. a The virus displaying RBD produced antibodies that can bind both RBD and gp64 protein at 10¹⁰ pfu/ml in a serum sample obtained from mice injected with AcRBD-sfGFP-V subcutaneously without any adjuvant. b Western blot of the AcRBD-sfGFP-64, AcRBD-sfGFP-V, and the AcDH10Bac detecting differences between the RBD-sfGFP and gp64 bands. Two distinguished bands corresponding to gp64 (~67 kDa) and RBD-sfGFP (~ 51 kDa) were observed in both AcRBD-sfGFP-64 and AcRBD-sfGFP-V samples but only a single band corresponding to gp64 was observed in the AcDH10Bac