Fig. 2

The phylogenetic analysis of HI03-3b 16S-rRNA gene sequence using the UPGMA method [43]. The bootstrap consensus tree inferred from 1000 replicates represents the evolutionary history of the taxa analyzed [19]. The evolutionary distances were calculated according to the maximum composite likelihood method [62]. Evolutionary analyses were performed in MEGA X [32], which involved 45 16S-rRNA gene sequences from representative members of some genera within Bacillaceae family. There were a total of 1593 positions in the final dataset. The phylogenetic tree covers representative members of some genera within Bacillaceae family (Bacillus, Cytobacillus, Mesobacillus, Neobacillus, and Peribacillus). Neobacillus clade is as follows: 1, N. bataviensis NBRC 102449; 2, N. drentensis IDA1967; 3, N. novalis NBRC 102450; 4, N. niacini NBRC 15566; and 5, N. pocheonensis Gsoil 420. Mesobacillus clade is as follows: 1, M. foraminis CV53; 2, M. zeae JJ-247; 3, M. campisalis SA2-6; 4, M. stamsii BoGlc83; 5, M. thioparans BMP-1; 6, M. boroniphilus T-15Z; 7, M. subterraneus COOI3B; and 8, M. jeotgali YKJ-10. Peribacillus clade is as follows: 1, P. kribbensis BT080; 2, P. cavernae L5; 3, P. asahii A001; 4, P. psychrosaccharolyticus 23296; 5, P. muralis LMG 20238; 6, P. frigoritolerans DSM 8801; 7, P. implex NBRC 15720; and P. simplex LMG 11160. Bacillus clade is as follows: 1, B. aerophilus 28K; 2, B. stratosphericus 1KF2a; 3, B. licheniformis DSM 13; 4, B. licheniformis ATCC 14580; 5, B. velezensis FZB42; 6, B. vallismortis DSM 11031; 7, B. subtilis IAM 12118; 8, B. inaquosorum BGSC 3A28; 9, B. halotolerans DSM 8802; and 10, B. niacini IFO15566