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Journal of Genetic Engineering and Biotechnology
Fig. 4 | Journal of Genetic Engineering and Biotechnology

Fig. 4

From: Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

Fig. 4

Molecular analysis of embryogenic calli in transgenic oil palm. Three days after co-bombardment of pAMdsRED with pLYCRISPRCas9P35S-H: EgFAD2 (a), pLYCRISPRCas9PUbi-H: EgPAT (b), and pLYCRISPRCas9PUbi-H: EgFAD2EgPAT (c), DsRED fluorescent spots on calli were observed. The scale bar is 200 µm. PCR analysis using cDNA (d) to detect the expression of Cas9 with (e) Actin as an internal control to confirm the quality and integrity of the cDNAs. pLYCRISPRCas9P35S-H: EgFAD2, pLYCRISPRCas9PUbi-H: EgPAT, and pLYCRISPRCas9PUbi-H: EgFAD2EgPAT are found in lanes 1–6, 7–12, and 13–18. The results showed that Cas9 gene expression was detected, as indicated by 560 bp PCR products. Lane M is a 1-kb plus DNA ladder (Invitrogen)

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