Cultivation of Pseudochlorella pringsheimii for biodiesel production in a scalable indoor photobioreactor: case studies from Egypt

Journal of Genetic Engineering and Biotechnology

Table 7 Physiochemical properties of Pseudochlorella pringsheimii biodiesel, diesel fuel and biodiesel standard

Property	Units	Biodiesel of microalgae	Diesel^a Fuel	ASTM^a Biodiesel Standard
Density	(Kg⁻¹L)		0.838	0.86—0.9
Viscosity	mm² S⁻¹, at 40 ºC	4.98 ± o.24	1.9—4.1	3.5—5.0
Acid value (AV)	mg KOH ⁻¹ g	0.41 ± 0.09	Max 0.5	Max 0.5
Peroxide number	meq/kg	ND	ND
saponification value (SV)	(mg of KOH/g)	199.33 ± 1.66	….	………
Iodine value	g I₂/100 g	89.84 ± 3.33	120	Max 120
Cetane number (CN)		53.46	45.9	≥ 47
Oxidiz ability (OX)		0.169	……	> 0.7
DU		97.29	……	……
Heating value	(MJ/kg) HV	39.22
HHV		41.75
Flash point (FP)	(°C)	79.5		93
FP		45.87
LCSF		31.42		….
CFPP		82.25		……

^aThe data about diesel and ASTM biodiesel standard were taken from published literature
DU = MUFA + (2X PUSFA)
CN = (46.3 + [5458/SV]) -(0.225 X IV)
Oxidize ability (OX) = [0.02(%Oleic) + % linoleic + 2 (% linolenic)] / 100
Higher Heating Value (HHv) = 0.4625 V (viscosity) + 39.450
Cloud point (CP), = (HHV -32.12) /0.21
Long-chain saturated factor (LCSF) = (0.1 X C₁₆) + (0.5 XC₁₈) + (1 XC₂₀) + (1.5 X C₂₂) + (2 X C₂₄)
Long-chain saturated factor (LCSF) = (0.1 X C₁₆) + (0.5 XC₁₈) + (1 XC₂₀) + (1.5 X C₂₂) + (2 X C₂₄)