In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

Journal of Genetic Engineering and Biotechnology

Table 3 Effect of chitosan (CHT) and thidiazuron (TDZ) on a response percentage (%) of callus for bud formation, and a number of buds/100 mg callus for date palm, cv. Barhee after 12 weeks of culturing

Treatments (mgL⁻¹)	frequency [%]	Shoot number	Treatments(mgL⁻¹)	frequency [%]	Shoot number
0.0 CHT + 0.0 TDZ	0.0 ± 0.0	0.0 ± 0.0	10 CHT + 1.0 TDZ	41.67 ± 3.89	6.60 ± 0.58
0.0 CHT + 0.5 TDZ	25.0 ± 4.81	3.0 ± 0.50	15 CHT + 0.0 TDZ	25.0 ± 4.81	3.33 ± 0.4
0.0 CHT + 1.0 TDZ	16.67 ± 1.53	2.5 ± 0.22	15 CHT + 0.5 TDZ	75.00 ± 4.60	16.11 ± 0.99
5 CHT + 0.0 TDZ	8.34 ± 1.21	2.0 ± 0.19	15 CHT + 1.0 TDZ	58.34 ± 4.81	13.2 ± 0.59
5 CHT + 0.5 TDZ	41.67 ± 3.89	7.4 ± 0.58	20 CHT + 0.0 TDZ	25.0 ± 4.81	3.66 ± 0.42
5 CHT + 1.0 TDZ	33.34 ± 3.05	6.25 ± 0.4	20 CHT + 0.5 TDZ	58.34 ± 4.81	11.14 ± 0.17
10 CHT + 0.0 TDZ	16.67 ± 1.53	2.5 ± 0.22	20 CHT + 1.0 TDZ	41.67 ± 3.89	9.2 ± 0.21
10 CHT + 0.5 TDZ	58.34 ± 4.81	7.85±0.1
LSD < 0.05	13.9	0.7		13.9	0.7