Table 1 Influence of cytokinins supplemented to Murashige and Skoog medium on In vitro regeneration of shoots from Andrographis producta using nodal and shoot tip explants

Control

Node

66.66

Shoot tip

83.33

Node

1.25 ± 0.25d

Shoot tip

1.00 ± 0.00f

Node

0.85 ± 0.13fgh

Shoot tip

1.32 ± 0.13e

KN

 2.5

83.33

33.33

1.60 ± 0.24d

1.00 ± 0.00f

1.79 ± 0.14a

1.50 ± 0.00de

 5.0

66.66

50

1.25 ± 0.25d

1.00 ± 0.00f

0.70 ± 0.07gh

1.53 ± 0.14de

 7.5

50

66.66

1.00 ± 0.00d

1.00 ± 0.00f

0.80 ± 0.05gh

1.30 ± 0.17e

 10

50

50

1.00 ± 0.00d

1.00 ± 0.00f

0.60 ± 0.10 h

1.76 ± 0.03 cd

BAP

 2.5

66.66

83.33

2.25 ± 0.25d

10.40 ± 0.50e

0.71 ± 0.04gh

2.23 ± 0.12ab

 5.0

66.66

100

5.75 ± 0.62c

17.50 ± 0.88c

0.90 ± 0.12efgh

2.45 ± 0.08a

 7.5

83.33

83.33

7.00 ± 0.83c

14.00 ± 0.54d

1.03 ± 0.06cdefg

1.97 ± 0.08bc

 10

83.33

83.33

9.60 ± 0.50b

10.00 ± 0.77e

1.35 ± 0.04bc

1.76 ± 0.05 cd

TDZ

 2.5

83.33

50

1.40 ± 0.24d

1.00 ± 0.00f

0.94 ± 0.08defgh

0.90 ± 0.05f

 5

50

50

1.00 ± 0.00d

1.00 ± 0.00f

0.86 ± 0.06fgh

0.86 ± 0.06f

 7.5

50

33.33

1.00 ± 0.00d

1.00 ± 0.00f

0.76 ± 0.08gh

1.20 ± 0.10ef

 10

50

33.33

1.00 ± 0.00d

1.00 ± 0.00f

1.16 ± 0.08bcdef

1.30 ± 0.10e

2-iP

 2.5

66.66

100

2.00 ± 0.00d

16.16 ± 0.65 cd

1.22 ± 0.21bcde

2.11 ± 0.10abc

 5.0

83.33

100

6.80 ± 0.80c

20.50 ± 1.02b

0.94 ± 0.06defgh

2.06 ± 0.12abc

 7.5

83.33

100

9.20 ± 0.58b

21.50 ± 0.50b

1.24 ± 0.07bcd

2.03 ± 0.14bc

 10

83.33

100

14.60 ± 0.74a

27.66 ± 1.05a

1.40 ± 0.08b

1.90 ± 0.07bcd