Fig. 1

In vitro shoot regeneration from nodal and shoot tip explants of Andrographis producta on Murashige and Skoog medium supplemented with cytokinins. A Induction of axillary shoots from the nodal explant on MS medium supplemented with 10 μM 2-iP after 2 weeks of culture (bar-, 0.20 cm). B Shoot multiplication from nodal explant on MS medium supplemented with 10 μM 2-iP after 4 weeks (bar-, 0.23 cm). C Shoot proliferation from nodal explant on MS medium containing 10 μM 2-iP after 6 weeks (bar-, 0.35 cm). D Multiple shoots regeneration from nodal explant after 8 weeks of culture on medium supplemented with 10 μM 2-iP (bar-, 0.30 cm). E In vitro seed germination on 1/10th strength MS basal medium (bar-, 0.7 cm). F Initiation of multiple shoots from shoot tip explant on MS medium supplemented with 5 μM BAP after 2 weeks (bar-, 0.75 cm). G Proliferation of shoots from shoot tip explant on medium supplemented with 5 μM BAP after 4 weeks (bar-, 0.9 cm). H Multiple shoots regeneration from shoot tip explant after 8 weeks of culture on MS medium supplemented with 5 μM BAP (bar-, 0.98 cm). I Initiation of direct shoot buds from cut end region (basal region) of shoot tip explant after 2 weeks of culture on MS medium containing 10 μM 2-iP (bar-, 0.60 cm). J and K Proliferation of shoots from cut end region (basal region) on shoot tip explant on MS medium containing 10 μM 2-iP after 4 and 6 weeks, respectively (bar-, 0.55 cm and 1.15 cm for J and K, respectively). L Multiple shoots regenerated from a cut end region of shoot tip explant after 8 weeks of culture on MS medium supplemented with 10 μM 2-iP (bar, -1.03 cm)