Table 4 Statistical analysis of BB design showing coefficients and t and p values for significant variables affecting the CHO by Streptomyces sp. strain AN

Intercept

6.40523

0.086412

74.12

2.42E−17

2.064964

X1&RS

0.151137

0.043206

3.5

0.0044

99.56026

X2&RS

0.108563

0.043206

2.51

0.0273

97.27277

X3&RS

0.019158

0.043206

0.44

0.6654

33.46475

X4&RS

− 0.0479

0.043206

− 1.11

0.2894

71.06497

X1*X2

− 0.04231

0.074835

− 0.57

0.5823

41.77478

X1*X3

0.104572

0.074835

1.4

0.1876

81.2398

X2*X3

0.051089

0.074835

0.68

0.5078

49.22296

X1*X4

0.019158

0.074835

0.26

0.8023

19.7719

X2*X4

− 0.28498

0.074835

− 3.81

0.0025

99.75072

X3*X4

0.059869

0.074835

0.8

0.4392

56.07516

X1*X1

− 0.09739

0.064809

− 1.5

0.1588

84.12301

X2*X2

− 0.16604

0.064809

− 2.56

0.0249

97.50889

X3*X3

− 0.10856

0.064809

− 1.68

0.1197

88.02517

X4*X4

− 0.14209

0.064809

− 2.19

0.0488

95.12073

ANOVA

df

SS

MS

F

Prob > F

Model

14

0.102921

0.007352

3.3459

0.0213

Error

12

0.026366

0.002197

C. total

26

0.129287

R²

0.796068

R² Adj

0.558148

Root mean square error

0.046874

Mean of response

1.934444

Observations (or sum Wgts)

27