Table 1 Primers sequence designed for RT-PCR gene expression
Primer name | Primer sequence |
|---|---|
P53 | 5′-CAGCGTGA TGATGGTAAGGA-′3 5′-GCGTTGCTCTGATGGTGA-′3 |
Bcl2 | 5′-TCTTCAGGCTGGAAGGAGAA-′3 5′-AAGCTGTCACAGAGGGGCTA-′3 |
Bax | 5′-GATCAGCTCGGGCACTTTAG-′3 5′-TGTTTGCTGATGGCAACTTC-′3 |
GAPDH | 5′-CCCTTCATTGACCTCAACTACAATGGT-′3 5′-GAGGGGCCATCCACAGTCTTCTG-′3 |
SiRT-1 | 5′-TGGCAAAGGAGCAGATTAGTAGG-3′ 5′-CTGCCACAAGAACTAGAGGATAAGA-3′ |
ERDJ-4 | 5′-AGTAGACAAAGGCATCATTTCCAA-3′ 5′-CTGTATGCTGATTGGTAGAGTCAA-3′ |
BNP | 5′-GCAGAAGCTGCTGGAGCTGA-3′ 5′-GATCCGGAAGGCGCTGTCT-3′ |