Table 1 List of random amplification of polymorphic DNA (RAPD) primers, their sequence motifs, total number, and size of the amplified loci used for determination of genetic integrity of hardened and mother plants of Artocarpus heterophyllus L.
Sl no | Primer code | Primer sequence (5’-3’) | Number of amplified loci | Total number of bands amplified | Number of polymorphic band | Range of amplification (bp) |
|---|---|---|---|---|---|---|
1 | OPS-01 | AAATCGGAGC | 2 | 24 | 0 | 750–1500 |
2 | 0PS-02 | GGTCCCTGAC | 3 | 36 | 0 | 300–1200 |
3 | OPS-03 | GTCCTACTCG | 2 | 24 | 0 | 800–1480 |
4 | OPS-04 | TGCGCGATCG | 3 | 36 | 0 | 400–1500 |
5 | OPS-05 | AGTCAGCCAC | 4 | 48 | 0 | 300–2000 |
6 | OPS-06 | AATCGGGCTG | 2 | 24 | 0 | 700–1500 |
7 | OPS-07 | AACGTACGCG | 4 | 48 | 0 | 300–2000 |
8 | OPS-08 | GCACGCCGGA | 2 | 24 | 0 | 800–1500 |
9 | OPS-09 | AGGGGTCTTG | 5 | 60 | 0 | 500–2000 |
10 | OPS-10 | TTATCTTGAC | 2 | 24 | 0 | 450–1350 |
11 | OPS-11 | GGGTCTCGGT | 4 | 48 | 0 | 300–2000 |
12 | OPS-12 | CGGTTGGTTA | 3 | 36 | 0 | 250–600 |
13 | OPS-13 | AGAGCCGTCA | 2 | 24 | 0 | 300–1000 |
14 | OPS-14 | CGGTATTATGC | 5 | 60 | 0 | 550–1000 |
15 | OPS-15 | AGCCGTGGAA | 6 | 72 | 0 | 100–2500 |
Total | 49 | 588 | Â | Â | ||
Average | 3.27 | 39.2 | Â | Â | ||