From: Enhancement of effector functions of anti-CD20 monoclonal antibody by increased afucosylation in CHO cell line through cell culture medium optimization
Glycans (%)
Before adding 8X UMG
After adding 8X UMG
Total galactose
44.24
39.80
Total mannose
7.70
8.43
Total fucosylation
82.00
71.8
Total afucose
5.42
13.12
Osmolality on day 11
440 mOsm
326 mOsm