Table 1 Biochemical test and enzyme assay
No | Type of assay | Bacillus sp. LPPI-18 | Bacillus cereus ATCC 14579T (Boone et al., 2001; de Vries et al., 2004; Ivanova et al., 2003) |
|---|---|---|---|
1 | Sample source | Soil sediment | Dairy |
2 | Morphology | Short rod | Rod shaped |
3 | Temperature for growth | 25-37oC | 10-45 oC |
4 | Fresh plate culture | No smell | Mousy smell |
5 | 2M NaCl | - | NP |
6 | Colony color | White | White to cream and sometimes Pinkish brown |
7 | 1.5 M NaCl | - | NP |
8 | pH range | 7-11 | 7 |
9 | Gram staining | + | + |
10 | Endospore | + | + |
11 | Citrate test | - | + |
12 | TSI test (H2S production) | + | - |
13 | Bile salt | - | + |
14 | Methyl red test | + | + |
15 | Enzyme | Â | Â |
15.1 | Amylase test | + | - |
15.2 | Catalase test | + | + |
15.3 | Pectinase test | - | + |
15.5 | Protease test | + | + |
16 | Gelatinase | - | + |
17 | D-fructose | + | - |
18 | D-glucose | + | - |
19 | D-ribose | + | + |
20 | Glycerol | - | + |
21 | PHA production on Glycerol | - | NP |
22 | PHA production on Crude oil waste | - | NP |
23 | PHA production on Rice Bran | - | NP |
23 | PHA production on Wheat bran | - | NP |