Table 3 The PCR reaction parameters of ISSR and SCoT
Step | Temperature | Time period | Cycles | ||
|---|---|---|---|---|---|
SCoT | ISSR | SCoT | ISSR | ||
Initial denaturation | 94°C | 94°C | 5 min | 5 min | 1 |
Denaturation | 94°C | 94°C | 40 s | 40 s |  |
Annealing | 50°C | 45°C | 1 min | 1 min | 40 |
Extension | 72°C | 72°C | 1.5 min | 1.5 min |  |
Final extension | 72°C | 72°C | 7 min | 7 min | 1 |