Genome-wide identification, phylogeny, and expression profiling analysis of shattering genes in rapeseed and mustard plants

Journal of Genetic Engineering and Biotechnology

Table 1 Primers used for qRT-PCR analysis of shattering genes in B. juncea and B. napus fresh and mature siliques

Sr. no	Primer name	Primer sequences 5′–3′	Product size
1	SHP1-F	GTAGTCACGACGCAGAGAGTA	80
1	SHP1-R	AACTTCAGCATCACACAAGAC	80
2	SHP2-F	GTGTAAGAGGAACGATCGAAA	81
2	SHP2-R	TCACCAAGAATGTGTCTGTTC	81
3	FUL-F	GACTCTTGCATGGAAAGCATA	82
3	FUL-R	TCTTCTCAAGTACCTCAACTC	82
4	IND-F	GAAACCCTAAGCCACTTCCAG	81
4	IND-R	CTCGCTTATCCTTTCTCTAC	81
5	NAC-F	GGGCAGCAACTTCTGGTTACT	85
5	NAC-R	TCAGTGAGGCGATATTCATGC	85
6	ALC-F	GTTTCCTCCGCTGAGATGTTC	81
6	ALC-R	ATGAATTTCGCTGTCTAGCTC	81
7	RPL-F	GTGTGGGTCATGGTATTTACA	84
7	RPL-R	ATACCTCTTGTAAACCTCGTC	84
8	PG-F	GTGTGGAAGTCTCTCCAAATC	84
8	PG-R	ACACAGAGGGAGTAGCTTGCC	84
9	EF	CCAAGAATGGGCTTTATGC	130
9		GTGATAGAGTGTCCAACAAGGTAAGTA