S.N. | ISSR primers | Sequence (5’ – 3’) | GC-content | Annealing temperature | Total bands | Range (bp) | Polymorphism information content (PIC) | Discriminating power (DP) | Resolving power (RP) | Effective multiplex ratio (EMR) | Marker index (MI) |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | UBC 807 | (AG)8T | 47 | 51°C | 12 | 153-883 | 0.407 | 0.527 | 5.778 | 8.26 | 3.36 |
2 | UBC 808 | (AG)8C | 53 | 51°C | 14 | 187-941 | 0.401 | 0.891 | 7.704 | 4.63 | 1.85 |
3 | UBC 809 | (AG)8G | 53 | 51°C | 13 | 168-1499 | 0.375 | 0.780 | 7.926 | 5.63 | 2.11 |
4 | UBC 810 | (GA)8T | 47 | 45°C | 10 | 225-1434 | 0.414 | 0.495 | 5.185 | 7.11 | 2.95 |
5 | UBC 811 | (GA)8C | 53 | 45°C | 11 | 141-892 | 0.376 | 0.704 | 6.222 | 5.44 | 2.04 |
6 | UBC 813 | (CT)8T | 47 | 49°C | 12 | 324-2265 | 0.374 | 0.779 | 7.148 | 5.65 | 2.12 |
7 | UBC 818 | (CA)8G | 53 | 45°C | 8 | 162-1238 | 0.376 | 0.694 | 4.852 | 4.43 | 1.67 |
Average | 50.43 | 48.14 | 11.42 | 583.38 | 0.389±0.017 | 0.696±0.142 | 6.402±1.216 | 5.88±1.36 | 2.30±0.62 |