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Journal of Genetic Engineering and Biotechnology

Table 2 Depiction of primers used in the study and their amplification efficacy

From: Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

S.N.

ISSR primers

Sequence (5’ – 3’)

GC-content

Annealing temperature

Total bands

Range (bp)

Polymorphism information content (PIC)

Discriminating power (DP)

Resolving power (RP)

Effective multiplex ratio (EMR)

Marker index (MI)

1

UBC 807

(AG)8T

47

51°C

12

153-883

0.407

0.527

5.778

8.26

3.36

2

UBC 808

(AG)8C

53

51°C

14

187-941

0.401

0.891

7.704

4.63

1.85

3

UBC 809

(AG)8G

53

51°C

13

168-1499

0.375

0.780

7.926

5.63

2.11

4

UBC 810

(GA)8T

47

45°C

10

225-1434

0.414

0.495

5.185

7.11

2.95

5

UBC 811

(GA)8C

53

45°C

11

141-892

0.376

0.704

6.222

5.44

2.04

6

UBC 813

(CT)8T

47

49°C

12

324-2265

0.374

0.779

7.148

5.65

2.12

7

UBC 818

(CA)8G

53

45°C

8

162-1238

0.376

0.694

4.852

4.43

1.67

Average

50.43

48.14

11.42

583.38

0.389±0.017

0.696±0.142

6.402±1.216

5.88±1.36

2.30±0.62

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