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Table 2 Depiction of primers used in the study and their amplification efficacy

From: Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

S.N. ISSR primers Sequence (5’ – 3’) GC-content Annealing temperature Total bands Range (bp) Polymorphism information content (PIC) Discriminating power (DP) Resolving power (RP) Effective multiplex ratio (EMR) Marker index (MI)
1 UBC 807 (AG)8T 47 51°C 12 153-883 0.407 0.527 5.778 8.26 3.36
2 UBC 808 (AG)8C 53 51°C 14 187-941 0.401 0.891 7.704 4.63 1.85
3 UBC 809 (AG)8G 53 51°C 13 168-1499 0.375 0.780 7.926 5.63 2.11
4 UBC 810 (GA)8T 47 45°C 10 225-1434 0.414 0.495 5.185 7.11 2.95
5 UBC 811 (GA)8C 53 45°C 11 141-892 0.376 0.704 6.222 5.44 2.04
6 UBC 813 (CT)8T 47 49°C 12 324-2265 0.374 0.779 7.148 5.65 2.12
7 UBC 818 (CA)8G 53 45°C 8 162-1238 0.376 0.694 4.852 4.43 1.67
Average 50.43 48.14 11.42 583.38 0.389±0.017 0.696±0.142 6.402±1.216 5.88±1.36 2.30±0.62