Fig. 8

Impact of imidazole elution on IgG binding. Direct ELISA assays using 50 ng immobilized human serum or 50 ng BSA, followed by SA-TRIM21-HRP (0.5 μg) or HRP-coupled Protein A/G incubation and washing with imidazole-containing buffers. Chromogenic substrate TMB was added to the wells, incubated for 10 min, followed by the addition of 50 μL H 2SO4 and OD450 reading. The data shows the ratio of serum/control OD450 reading. Experiments were performed in triplicates, and error bars represent standard deviation