Fig. 5
From: TRIM21 chimeric protein as a new molecular tool for multispecies IgG detection
Performance of SA-TRIM21 for IgG detection on veterinary ELISA kits: Anti-Leishmania IgG detection in dog serum. Wells were coated with specific L. infantum antigens, followed by incubation with dog serum samples. Wells were washed followed by incubation with HRP coupled anti-IgG or SA-TRIM21. After washing, the chromogen TMB was added to the wells followed by incubation during 10 min. Reactions were quenched using Stop solution and absorbance was read at 450 nm