Table 1 Biochemical test and enzyme assay
No | Type of assay | Bacillus sp. LPPI-18 | |
|---|---|---|---|
1 | Isolate source | Soil sediment | Dairy |
2 | Morphology | Short rod | Rod shaped |
3 | Temperature for growth | 25–37 °C | 10–45 °C |
4 | Fresh plate culture | No smell | Mousy smell |
5 | 2M NaCl | – | NP |
6 | Colony color | White | White to cream and sometimes pinkish brown |
7 | 1.5 M NaCl | – | NP |
8 | pH range | 7–11 | 7 |
9 | Gram staining | + | + |
10 | Endospore | + | + |
11 | Citrate test | + | + |
12 | TSI test (H2S production) | + | – |
13 | Bile salt | + | + |
14 | Methyl red test | + | + |
15 | Enzyme | Â | Â |
15.1 | Amylase test | – |  |
15.2 | Catalase test | + | + |
15.3 | Pectinase test | + | + |
15.5 | Protease test | + | + |
16 | Gelatinase | – | + |
17 | D-fructose | + | – |
18 | D-glucose | + | – |
19 | D-ribose | + | + |
20 | Glycerol | – | + |
21 | PHA production on glycerol | – | NP |
22 | PHA production on crude oil waste | – | NP |