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Table 1 Biochemical test and enzyme assay

From: Polyhydroxyalkanoate recovery from newly screened Bacillus sp. LPPI-18 using various methods of extraction from Loktak Lake sediment sample

No Type of assay Bacillus sp. LPPI-18 Bacillus cereus ATCC 14579T [24,25,26]
1 Isolate source Soil sediment Dairy
2 Morphology Short rod Rod shaped
3 Temperature for growth 25–37 °C 10–45 °C
4 Fresh plate culture No smell Mousy smell
5 2M NaCl NP
6 Colony color White White to cream and sometimes pinkish brown
7 1.5 M NaCl NP
8 pH range 7–11 7
9 Gram staining + +
10 Endospore + +
11 Citrate test + +
12 TSI test (H2S production) +
13 Bile salt + +
14 Methyl red test + +
15 Enzyme   
15.1 Amylase test  
15.2 Catalase test + +
15.3 Pectinase test + +
15.5 Protease test + +
16 Gelatinase +
17 D-fructose +
18 D-glucose +
19 D-ribose + +
20 Glycerol +
21 PHA production on glycerol NP
22 PHA production on crude oil waste NP
  1. MA MacConkey agar, LPPI Loktak Lake phumula PHA isolates