In silico mutational analysis to identify the role and pathogenicity of BCL-w missense variants

Kumari, Poonam; Rameshwari, Rashmi

doi:10.1186/s43141-022-00389-2

Journal of Genetic Engineering and Biotechnology

Table 1 Stability predictions of missense variants using various prediction tools by using fasta format as input

From: In silico mutational analysis to identify the role and pathogenicity of BCL-w missense variants

S.No	Missense mutations	I-Mutant2.0	MUpro	SAAFEC	IStable
1	A159V	0.78 Decrease	− 0.383 Decreasing	− 0.04 Destabilizing	Increase
2	G154W	– 1.56 Decrease	− 0.332 Decreasing	− 0.38 Destabilizing	Increase
3	R161H	− 0.73 Decrease	− 1.345 Decreasing	− 0.80 Destabilizing	Decrease
4	E146K	− 1.11 Decrease	− 1.300 Decreasing	− 0.57 Destabilizing	Decrease
5	L180Q	− 2.55 Decrease	− 1.839 Decreasing	− 1.68 Destabilizing	Decrease
6	V178M	− 3.82 Decrease	− 0.277 Decreasing	− 0.83 Destabilizing	Increase
7	A177P	0.87 Decrease	− 1.188 Decreasing	− 0.95 Destabilizing	Decrease
8	S169P	0.36 Increase	− 1.818 Decreasing	− 0.02 Destabilizing	Decrease
9	A159P	− 0.61 Decrease	− 1.71 Decreasing	− 0.41 Destabilizing	Decrease
10	A7T	− 0.97 Decrease	− 0.700 Decreasing	− 0.65 Destabilizing	Decrease
11	A7G	− 0.98 Decrease	− 1.108 Decreasing	− 0.70 Destabilizing	Decrease
12	A7V	0.89 Increase	− 0.458 Decreasing	− 0.64 Destabilizing	Increase
13	P8L	0.57 Increase	− 0.546 Decreasing	− 0.54 Destabilizing	Decrease
14	A15T	− 1.37 Decrease	− 1.302 Decreasing	− 0.85 Destabilizing	Decrease
15	D16H	− 1.53 Decrease	− 2.114 Decreasing	− 0.22 Destabilizing	Decrease
16	R23K	− 1.38 Decrease	− 0.717 Decreasing	− 0.74 Destabilizing	Decrease
17	G34W	− 1.01 Decrease	0.524 Increase	− 0.70 Destabilizing	Increase
18	M46T	− 0.80 Decrease	− 1.556 Decreasing	− 2.46 Destabilizing	Decrease
19	M46I	0.25 Increase	− 0.826 Decreasing	− 1.11 Destabilizing	Increase
20	R47Q	− 0.07 Decrease	− 0.786 Decreasing	− 1.06 Destabilizing	Increase
21	G50R	− 0.24 Decrease	− 1.055 Decreasing	− 0.76 Destabilizing	Increase
22	G50V	− 0.00 Increase	− 1.074 Decreasing	− 1.05 Destabilizing	Decrease
23	E54K	− 1.96 Decrease	− 1.066 Decreasing	− 0.59 Destabilizing	Decrease
24	F57S	− 1.69 Decrease	− 2.031 Decreasing	− 2.68 Destabilizing	Decrease
25	R58Q	− 0.45 Decrease	− 0.878 Decreasing	− 0.71 Destabilizing	Decrease
26	R59C	− 0.29 Decrease	− 1.086 Decreasing	− 0.49 Destabilizing	Decrease
27	R59H	− 0.90 Decrease	− 1.488 Decreasing	− 0.58 Destabilizing	Decrease
28	S62F	0.30 Increase	− 0.682 Decreasing	− 0.36 Destabilizing	Increase
29	A66D	− 0.23 Decrease	− 0.766 Decreasing	− 0.66 Destabilizing	Increase
30	P72T	− 0.88 Decrease	− 0.976 Decreasing	− 1.10 Destabilizing	Decrease
31	S74L	2.04 Increase	0.475 Increasing	− 0.48 Destabilizing	Increase
32	Q76K	− 0.13 Increase	− 0.978 Decreasing	− 0.60 Destabilizing	Decrease
33	R78H	− 1.33 Decrease	− 0.917 Decreasing	− 0.86 Destabilizing	Decrease
34	S83F	1.35 Increase	− 0.158 Decreasing	− 0.67 Destabilizing	Increase
35	D84N	0.36 Increase	− 0.895 Decreasing	0.18 Stabilizing	Increase
36	N92Y	− 0.64 Decrease	0.137 Increasing	− 0.58 Destabilizing	Increase
37	R95S	− 1.76 Decrease	− 1.048 Decreasing	− 1.36 Destabilizing	Decrease
38	R95H	− 0.69 Decrease	− 1.092 Decreasing	− 1.11 Destabilizing	Decrease
39	S110R	− 0.39 Increase	− 0.722 Decreasing	− 0.80 Destabilizing	Increase
40	V111I	− 0.58 Decrease	− 0.480 Decreasing	− 0.33 Destabilizing	Decrease
41	V127M	− 1.16 Decrease	− 0.536 Decreasing	− 0.46 Destabilizing	Decrease
42	A128V	− 0.55 Decrease	− 0.296 Decreasing	0.09 Stabilizing	Decrease
43	E131G	− 0.84 Decrease	− 1.672 Decreasing	− 0.81 Destabilizing	Decrease
44	Q133R	− 0.06 Decrease	− 1.196 Decreasing	− 0.08 Destabilizing	Increase
45	A135V	− 0.77 Decrease	− 0.525 Decreasing	− 0.17 Destabilizing	Increase
46	S140C	− 0.23 Increase	− 0.575 Decreasing	− 0.26 Destabilizing	Increase
47	S141I	0.91 Increase	− 0.269 Decreasing	− 0.06 Destabilizing	Increase
48	G142E	− 1.09 Decrease	− 1.223 Decreasing	− 1.27 Destabilizing	Decrease
49	G152R	− 1.38 Decrease	− 0.671 Decreasing	− 0.94 Destabilizing	Decrease
50	R160W	− 0.67 Decrease	− 0.744 Decreasing	− 0.88 Destabilizing	Decrease
51	R161L	− 0.12 Decrease	− 0.316 Decreasing	− 0.44 Destabilizing	Decrease
52	R163W	− 0.55 Decrease	− 0.852 Decreasing	− 0.06 Destabilizing	Decrease
53	R171M	− 0.88 Decrease	− 0.328 Decreasing	− 0.44 Destabilizing	Decrease
54	V186A	− 3.07 Decrease	− 1.789 Decreasing	− 1.41 Destabilizing	Decrease
55	A188P	− 1.42 Increase	− 1.343 Decreasing	− 0.76 Destabilizing	Increase

Bold represents a destabilizing or decreased mutational effect by all the prediction tools used

Back to article page