Fig. 1

A: Unrooted phylogenetic tree of the DCL protein related to higher plants. B: The tree was rooted with the outlier (outgroup A. protothecoides DCL protein). The Phylogenetic relationship was inferred from full-length polypeptide sequences of the plant DCL proteins using the Maximum Likelihood method and JTT model [19] with log likelihood of − 18013.10. The percentage of trees in which the associated taxa clustered together in the bootstrap test (500 replicates) is shown as a symbol displayed on each branch (Felsenstein, 1985). Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the JTT model. Topology with superior log likelihood value was selected. A discrete gamma distribution was used to model evolutionary rate differences among sites (2 categories (+G, parameter = 1.6882)). The rate variation model allowed some sites to be evolutionarily invariable ([+I], 1.53% sites). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 275 polypeptide sequences from Eudicotyledons (242) and Liliopsida (31) and Klebsormidium nitens and A. protothecoides DCL polypeptide sequences used as outlier. All positions containing gaps and missing data were eliminated (complete deletion option). 131 positions in the final dataset was seen. Evolutionary analyses were conducted in MEGA11 and visualized by iTOL v5 online tool [24]