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Table 1 DNA-specific primers of the studied genes amplifications

From: Molecular docking and nucleotide sequencing of successive expressed recombinant fungal peroxidase gene in E.coli

Gene Primers Nucleotide sequence GC% Tm °C Product size
Peroxidase F-POX1 5-CCAAATCTCCTCTTCCTTA-3 42.1 56.8 211
R-POX2 5-TGTCCTCTAACACTTCTCGCA-3 47.6 58
URA F-URA3 5-GCAAGGGCTCCCTAGCTACT-3 60 60 175
R-URA3 5-AATGCGTCTCCCTTGTCATC-3 50 60.1
AmpR F-AmpR 5-GAGTATTCAACATTTCCG-3 52 61.6 179
R-AmpR 5-CGGGGCGAAAACTCT-3 65 36.2