Table 1 DNA-specific primers of the studied genes amplifications
Gene | Primers | Nucleotide sequence | GC% | Tm °C | Product size |
|---|---|---|---|---|---|
Peroxidase | F-POX1 | 5-CCAAATCTCCTCTTCCTTA-3 | 42.1 | 56.8 | 211 |
R-POX2 | 5-TGTCCTCTAACACTTCTCGCA-3 | 47.6 | 58 | ||
URA | F-URA3 | 5-GCAAGGGCTCCCTAGCTACT-3 | 60 | 60 | 175 |
R-URA3 | 5-AATGCGTCTCCCTTGTCATC-3 | 50 | 60.1 | ||
AmpR | F-AmpR | 5-GAGTATTCAACATTTCCG-3 | 52 | 61.6 | 179 |
R-AmpR | 5-CGGGGCGAAAACTCT-3 | 65 | 36.2 |