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Journal of Genetic Engineering and Biotechnology

Table 1 Purification of recombined HN protein

From: Expression and characterization of hemagglutinin–neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800

Fraction

Volume (ml)

Protein (mg)

Activity neuraminidase (unit)

Specific activity (pmol/min/mg)

Fold purification

Yield %

Cultural supernatant

200

40.6

63.34

1.5

1×

100

Pooled eluates from SP

20

29.15

62.66

2.14

1.4×

98

Pooled eluates from Ni-NTA

20

10.64

61.84

5.8

3.8×

97

  1. *Specific activity = (activity enzyme/protein)
  2. **Fold purification = (specific activity/starting special activity)
  3. ***Yield = [(activity enzyme/starting activity enzyme) × 100]
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