Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium

Journal of Genetic Engineering and Biotechnology

Table 1 Biochemical properties of Pedobacter sp. 3.14.7 keratinase in comparison with other keratinases reported in bibliography

Microorganism	Optimum pH	Optimum temperature (°C)	pH range	pH stability	Temperature stability (°C)	Inhibitors	Reference
Pedobacter sp. 3.14.7	7.5	55	6.0–8.0	7.0–10.0	30% residual activity (1 h at 55 °C)	EDTA phenanthroline	This work
Pedobacter cryoconitis	8.0	40	7.0–10.0	7.0–9.0	T1/2 30 min (40 °C)	EDTA phenanthroline	[32]
Bacillus zhangzhouensis	9.5	60	7.0–10.5	7.0–10.5	T1/2 120 min (60 °C)	Co⁺², Fe⁺², Hg⁺² PMSF	[29]
Bacillus pumilus	9.0	45	7.0–10.0	NR	NR	Zn⁺², Hg⁺², Pb⁺² PMSF	[11]
Ochrobactrum intermedium	9.0	40	4.0–9.0	9.0 (5h)	40–60 almost 2 h	Zn⁺², Fe⁺², Cu⁺², Mn⁺², Ag⁺², Hg⁺² PMSF, EDTA	[30]
Caldicoprobacter algeriensis	7.0	50	2.0–10.0	5.0–9.5	T1/2 120 h (50 °C)	Ni⁺², Hg⁺², Cd⁺² PMSF	[33]

NR not reported, t1/2 half-life of the enzyme, EDTA ethylenediaminetetraacetic acid, PMSF phenylmethylsulphonyl fluoride