Table 1 Physicochemical properties of SpPMO and other class B monooxygenases used in the study
Accession no. | Name of the protein | Organism | No of amino acids | MW (kDa) | pI | Instability index | Solubility index | Aliphatic index | GRAVY |
|---|---|---|---|---|---|---|---|---|---|
Putrescine monooxygenase | |||||||||
QBX90611.1 | SpPMO | Shewanella putrefaciens 95 | 505 | 57.24 | 6.46 | 40.56 | 0.143 | 78.85 | −0.359 |
ACK46161.1 | SbNMO | Shewanella baltica | 501 | 56.76 | 6.24 | 43.51 | 0.154 | 79.48 | −0.346 |
ADV54887.1 | Sp200NMO | Shewanella putrefaciens 200 | 505 | 57.21 | 6.37 | 40.16 | 0.147 | 78.48 | −0.359 |
ABP76132.1 | SpCN32NMO | Shewanella putrefaciens CN32 | 505 | 57.22 | 6.31 | 41.97 | 0.147 | 78.48 | −0.357 |
AOR50757.1 | GrNMO | Gordonia ruberipertincta | 438 | 48.84 | 5.03 | 38.24 | 0.394 | 89.66 | −0.17 |
AGJ55094.1 | StrNMO | Streptomyces | 428 | 48.26 | 5.37 | 37.81 | 0.245 | 79.39 | −0.356 |
CFO04355.1 | BpNMO | Bordetella pertussis | 478 | 54.95 | 7.32 | 47.78 | 0.081 | 80.86 | −0.489 |
Cadeverine monooxygenase | |||||||||
CBA23306.1 | EaNMO | Erwinia amylovora | 430 | 50.17 | 5.72 | 45.78 | 0.180 | 78.7 | −0.500 |
Lysine monooxygenases | |||||||||
WP_103556989.1 | EcNMO | Escherichia coli | 595 | 67.77 | 5.63 | 36.32 | 0.054 | 97.09 | −0.094 |
NP_216894.1 | MtNMO | Mycobacterium tuberculosis | 431 | 46.94 | 6.17 | 26.65 | 0.227 | 88.54 | −0.164 |
WP_099421877.1 | NfNMO | Nocardia farcinica | 456 | 49.71 | 7.94 | 33.01 | 0.273 | 89.23 | −0.246 |
Ornithine monooxygenases | |||||||||
NP_251076.1 | PaNMO | Pseudomonas aeruginosa | 443 | 49.48 | 6.03 | 39.44 | 0.134 | 90.32 | −0.252 |
WP_043726233.1 | KtzNMO | Kutzneria spp. | 424 | 47.29 | 5.51 | 40.11 | 0.412 | 78.63 | −0.325 |
XP_755103.1 | AfNMO | Aspergillus fumigates | 501 | 56.87 | 8.78 | 49.32 | 0.244 | 80.78 | −0.523 |
WP_039794392.1 | AlNMO | Amycolatopsis alba | 447 | 49.44 | 5.08 | 34.11 | 0.468 | 86.58 | −0.189 |
Flavin-containing monooxygenases | |||||||||
NP_001269621.1 | HsFMO | Homo sapiens | 536 | 60.81 | 6.58 | 36.29 | 0.289 | 87.09 | −0.053 |
AAM18566.2 | MaFMO | Methylophaga aminisulfidivorans | 456 | 52.99 | 5.13 | 33.34 | 0.469 | 65.26 | −0.543 |
Baeyer-Villiger monooxygenase | |||||||||
CAD10801.1 | XfBVMO | Xanthobacter flavus | 446 | 60.32 | 5.93 | 25.67 | 0.283 | 77.4 | −0.235 |