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Journal of Genetic Engineering and Biotechnology
Fig. 6 | Journal of Genetic Engineering and Biotechnology

Fig. 6

From: Production of biologically active recombinant buffalo leukemia inhibitory factor (BuLIF) in Escherichia Coli

Fig. 6

Expression of stem cell markers associated with buffalo embryonic stem cells. A The putative buffalo embryonic stem cell colonies were analysed for alkaline phosphatase activity. The undifferentiated stem cells exhibited alkaline phosphatase activity, images (1–3, and); 50 μm. B Morphology of buESCs colonies (1–3) after 5 passages in medium containing 40 ng/ml BuLIF (bright field; scale bar 50 μm) and immunocytochemistry with anti-Oct-4 (4), anti-Sox-2 (5), and anti-Nanog (6), antisera, respectively, showed presence of stem cell markers in the stem cells. Scale bar: 100 μm

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